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KMID : 0861020110260030023
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Korea Journal of Herbology
2011 Volume.26 No. 3 p.23 ~ p.29
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Anti-Inflammatory Effect of Aqueous Extract of Scolopendrae Corpus in RAW 264.7 Cells
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Cho Il-Joo
Choi Mee-Ok
Park Min-Chul
Song Ho-Joon
Park Sung-Joo
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Abstract
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Objective: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Scolopendrae Corpus (SC) on lipopolysaccharide (LPS)-induced inflammatory response.
Methods:To evaluate the anti-inflammatory effects of SC, we examined the inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines (TNF-a, inteleukin (IL)-1β and IL-6) on RAW 264.7 cells. We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and inhibitory kappa B a (Iχ-Ba) using western blot. Furthermore, we also investigated the effect of SC on LPS-induced endotoxin shock.
Results: Extract from SC itself had not any cytotoxic effect in RAW 264.7 cells. Aqueous extract from SC inhibited LPS-induced NO production and iNOS expression. SC pre-treatment also inhibited IL-1β, IL-6 production in RAW 264.7 cells. To investigate inhibitory effects of SC on inflammatory mediators, activation of MAPKs was examined. SC inhibited the phosphorylation of p38 kinases (p38), c-Jun NH₂-terminal kinase (JNK) and also the degradation of Iχ-Bα in RAW 264.7 cells stimulated with LPS. Furthermore, SC administration reduced LPS-induced endotoxin shock.
Conclusion: SC down-regulated LPS-induced production of inflammatory mediators through inhibition of activation of p38, JNK and degradation of Iχ-Bα. Taken together, our results suggest that SC may be a beneficial drug against inflammatory diseases such as sepsis.
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KEYWORD
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Lipopolysaccharide (LPS), Scolopendra subspinipes multilans, Inflammation, Cytokine
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